Codon-Optimized Expression and Purification of Truncated ORF2 Protein of Hepatitis E Virus in Escherichia coli
نویسندگان
چکیده
منابع مشابه
codon-optimized expression and purification of truncated orf2 protein of hepatitis e virus in escherichia coli
conclusions in this study, the optimized truncated orf2 protein was expressed in e. coli successfully and the highly purified protein was obtained, which can be a potential vaccine candidate and as an antigen in elisa to diagnose hev infections. results the subcloning was confirmed by pcr, restriction enzyme digestion, and dna sequencing of recombinant plasmid pet30a-orf2.2. the results obtaine...
متن کاملCodon-Optimized Expression and Purification of Truncated ORF2 Protein of Hepatitis E Virus in Escherichia coli
BACKGROUND Hepatitis E virus (HEV) is a causative agent of acute hepatitis among people of different age groups and has high mortality rate of up to 30% among pregnant women. Therefore, primary prevention of HEV infection is essential. OBJECTIVES The aim of this study was to obtain the highly purified truncated open reading frames 2 (ORF2) protein, which might be a future HEV vaccine candidat...
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Background: The UL31 protein of herpes simplex virus 1 (HSV-1) plays an important role in the HSV-1 replication, however, its pinpoint functions in the life cycle of the virus have yet to be adequately elucidated. Objectives: An antiserum specific for detecting HSV-1 UL31 was prepared as the foundation for future research on the role of UL31 in the course...
متن کاملCloning and expression of hepatitis E virus ORF2 as a vaccine candidate
Introduction: Hepatitis E virus (HEV) is a fecal-oral transmitting virus which causes a chronic liver disease. ORF2 is an immunogen capsid protein of HEV that has been proposed to be used for Hepatitis E vaccine design. It is a 660-amino acid protein which includes an immunogenic region (residues 112-607). This protein has been expressed in complete and truncated forms, using different expressi...
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conclusions the cell lines obtained in this work secreted potent, stable and specific anti-bv mabs, which were suitable for the development of herpes b virus diagnosis reagents. background herpes b virus (bv) is a zoonotic disease caused by double-stranded enveloped dna virus with cercopithecidae as its natural host. the mortality rate of infected people could be up to 70% with fatal encephalit...
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ژورنال
عنوان ژورنال: Jundishapur Journal of Microbiology
سال: 2014
ISSN: 2008-3645,2008-4161
DOI: 10.5812/jjm.11261